RPA2 antibody (AA 34-254)

Catalog No. ABIN7874069

This Rabbit Polyclonal antibody specifically detects RPA2 in WB, ELISA, IF, IHC (p) and FACS. It exhibits reactivity toward Human.

Quick Overview for RPA2 antibody (AA 34-254) (ABIN7874069)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-RPA2 Antibody

Binding Specificity: AA 34-254

Purpose: RPA32 Antibody / RPA2

Purification: Affinity purified

Immunogen: Recombinant human protein (amino acids Q34-H254) was used as the immunogen for the RPA32 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RPA32 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RPA32 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Alternative Name: RPA32

Background: This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways.

UniProt: P15927

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA