IRAK1 antibody (pSer376)

Catalog No. ABIN7874123

This Rabbit Polyclonal antibody specifically detects IRAK1 in WB. It exhibits reactivity toward Human.

Quick Overview for IRAK1 antibody (pSer376) (ABIN7874123)

Target: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IRAK1 Antibody

Binding Specificity: AA 348-381, pSer376

Purpose: Phospho-IRAK1 Antibody (S376)

Purification: Antigen affinity

Immunogen: A portion of amino acids 348-381 from human IRAK1 was used as the immunogen for the phospho-IRAK1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the phospho-IRAK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the phospho-IRAK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for IRAK1

Target: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Alternative Name: IRAK1

Background: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor- signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3. [UniProt]

UniProt: P51617

Pathways: NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades