ITGA4 antibody (AA 35-269)

Catalog No. ABIN7874159

This Rabbit Polyclonal antibody specifically detects ITGA4 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for ITGA4 antibody (AA 35-269) (ABIN7874159)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITGA4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ITGA4 Antibody

Binding Specificity: AA 35-269

Purpose: ITGA4 Antibody / Integrin Alpha 4

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Integrin Alpha 4/ITGA4 recombinant protein (Position: N35-V269) was used as the immunogen for the ITGA4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ITGA4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ITGA4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: ITGA4

Background: Integrin alpha 4 antibody detects Integrin alpha-4 (encoded by ITGA4), a cell surface adhesion receptor that mediates interactions between cells and the extracellular matrix, primarily through binding to vascular cell adhesion molecule 1 (VCAM1) and fibronectin. Encoded by the ITGA4 gene on chromosome 2q31.3, this protein pairs with the beta-1 (ITGB1) or beta-7 (ITGB7) subunit to form functional integrin heterodimers such as alpha4beta1 (VLA-4) and alpha4beta7. These complexes regulate leukocyte adhesion, migration, and immune cell trafficking across vascular and epithelial barriers.

Integrin alpha-4 is a transmembrane glycoprotein composed of a large extracellular domain, a single transmembrane helix, and a short cytoplasmic tail that links to cytoskeletal and signaling molecules. It facilitates rolling and firm adhesion of lymphocytes and monocytes during inflammatory responses, guiding immune cells to sites of tissue injury or infection. In the nervous system, it contributes to axonal guidance and glial cell adhesion.

The ITGA4 antibody is widely used in immunology, oncology, and vascular biology research to study adhesion, immune cell migration, and integrin-mediated signaling. Flow cytometry and western blot analyses identify Integrin alpha-4 as a 150 kilodalton band, while immunohistochemistry reveals membrane staining on leukocytes, endothelium, and fibroblasts. This antibody provides a means to examine integrin expression dynamics and cell adhesion regulation under physiological and pathological conditions.

Integrin alpha-4 is clinically relevant as a therapeutic target in multiple sclerosis and Crohn's disease, where monoclonal antibodies blocking alpha4-integrin (e.g., natalizumab) reduce leukocyte infiltration and inflammation. Aberrant expression also contributes to cancer metastasis and fibrotic diseases by enhancing cell adhesion and migration. The ITGA4 antibody supports investigations into these mechanisms by detecting endogenous protein in tissues and cell lines. NSJ Bioreagents offers this antibody validated for its applications, ensuring specificity and sensitivity for integrin research.

UniProt: P13612

Pathways: Integrin Complex