TBKBP1 antibody (AA 35-467)

Catalog No. ABIN7874169

The Rabbit Polyclonal anti-TBKBP1 antibody is suitable to detect TBKBP1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF, FACS and ICC.

Quick Overview for TBKBP1 antibody (AA 35-467) (ABIN7874169)

Target: TBKBP1 (TBK1 Binding Protein 1 (TBKBP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TBKBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-TBKBP1 Antibody

Binding Specificity: AA 35-467

Purpose: TBKBP1 Antibody / TANK-binding kinase 1-binding protein 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TBKBP1 recombinant protein (Position: D35-E467) was used as the immunogen for the TBKBP1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TBKBP1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TBKBP1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TBKBP1

Target: TBKBP1 (TBK1 Binding Protein 1 (TBKBP1))

Alternative Name: TBKBP1

Background: TBKBP1 antibody recognizes TANK-binding kinase 1-binding protein 1, a scaffold protein that links TANK-binding kinase 1 (TBK1) and IKK epsilon to downstream effectors in antiviral and inflammatory signaling pathways. Encoded by the TBKBP1 gene, also known as SINTBAD (Similar to NAP1 TBK1 adaptor), this cytosolic adaptor protein participates in type I interferon responses by coordinating the assembly of signaling complexes involved in innate immunity. TBKBP1 interacts with kinases TBK1 and IKK epsilon to facilitate activation of interferon regulatory factors IRF3 and IRF7, leading to production of antiviral cytokines.

TBKBP1 functions as part of a network of adaptor proteins, including NAP1 and TANK, that integrate pattern recognition receptor (PRR) signaling from Toll-like receptors, RIG-I-like receptors, and cytosolic DNA sensors. Upon pathogen detection, TBKBP1 recruits TBK1 to perinuclear regions and promotes its activation via phosphorylation. This process is essential for host defense against viral infection. Beyond immune responses, TBKBP1 also participates in autophagy regulation and may influence NF-kappaB signaling, linking innate immune sensing with cell survival and metabolic adaptation.

The TBKBP1 gene is located on chromosome 17q21.31 and encodes a protein with coiled-coil and leucine zipper domains that mediate multimeric complex formation. Expression is observed in immune tissues, liver, and lung, and can be induced by viral infection or interferon stimulation. Structural studies suggest that TBKBP1 provides a flexible scaffold for recruitment of kinases and transcription factors, enabling precise control of signal propagation. Genetic studies have associated TBKBP1 polymorphisms with autoimmune disorders and susceptibility to chronic inflammation.

Immunohistochemical analysis using TBKBP1 antibody reveals cytoplasmic distribution in macrophages, dendritic cells, and epithelial tissues. The antibody is widely used to study antiviral defense mechanisms, cytokine signaling, and innate immune regulation. TBKBP1 antibody from NSJ Bioreagents provides an important reagent for investigating TBK1/IKK-related pathways and the molecular coordination of immune responses.

UniProt: A7MCY6

Pathways: SARS-CoV-2 Protein Interactome