PAPPA antibody (AA 351-523)

Catalog No. ABIN7874254

The Mouse Monoclonal anti-PAPPA antibody (Clone IDIP-1) (ABIN7874254) specifically detects PAPPA in IHC (p). The antibody is reactive with Human samples.

Quick Overview for PAPPA antibody (AA 351-523) (ABIN7874254)

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAPPA antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: IDIP-1

Product Details anti-PAPPA Antibody

Binding Specificity: AA 351-523

Purpose: PAPPA Antibody / Pappalysin-1

Purification: Protein G affinity chromatography

Immunogen: A portion of amino acids 351-523 from the human protein was used as the immunogen for the PAPPA antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the PAPPA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the PAPPA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for PAPPA

Target: PAPPA (Pregnancy-Associated Plasma Protein A, Pappalysin 1 (PAPPA))

Alternative Name: PAPPA

Background: The PAPPA gene encodes a secreted metalloproteinase which cleaves insulin-like growth factor binding proteins (IGFBPs). Following IGFBP cleavage, insulin growth factors dissociate from IGFBPs and bind to IGF receptors, resulting in activation of the IGF pathway. The encoded protein plays a role in bone formation, inflammation, wound healing and female fertility. Enhanced expression of this protein is associated with diabetic nephropathy in human patients and this protein may promote tumor invasion and growth in various human cancers. [RefSeq]

UniProt: Q13219