Melanoma gp100 antibody (AA 376-502)

Catalog No. ABIN7874468

This Mouse Monoclonal antibody specifically detects Melanoma gp100 in WB, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Melanoma gp100 antibody (AA 376-502) (ABIN7874468)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma gp100 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PMEL-2037

Product Details anti-Melanoma gp100 Antibody

Binding Specificity: AA 376-502

Purpose: PMEL17 Antibody / Melanoma gp100

Purification: Protein G affinity chromatography

Immunogen: A portion of amino acids 376-502 from the human protein was used as the immunogen for the PMEL17 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the PMEL17 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the PMEL17 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: PMEL17

Background: Cytotoxic T lymphocytes (CTL's) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein.

The gp100 Molecule is a 100 kDa glycosylated protein that is cleaved into a small (26 kDa) carboxy-terminal fragment and a larger amino- terminal section (60-64 kDa), which is subsequently cleaved to generate 26 kDa and 34-38 kDa fragments.

UniProt: P40967