WHSC2 antibody (AA 38-482)

Catalog No. ABIN7874510

This Rabbit Polyclonal antibody specifically detects WHSC2 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for WHSC2 antibody (AA 38-482) (ABIN7874510)

Target: WHSC2 (Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WHSC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-WHSC2 Antibody

Binding Specificity: AA 38-482

Purpose: NELFA Antibody / Negative elongation factor A

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human NELFA recombinant protein (Position: I38-L482) was used as the immunogen for the NELFA antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NELFA antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NELFA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for WHSC2

Target: WHSC2 (Wolf-Hirschhorn Syndrome Candidate 2 (WHSC2))

Alternative Name: NELFA

Background: NELFA antibody detects Negative elongation factor A, a transcriptional regulator that controls RNA polymerase II pausing and transcription elongation. Encoded by the NELFA gene on chromosome 9q34.11, this protein is a core component of the negative elongation factor (NELF) complex, which also includes NELFB, NELFC/D, and NELFE subunits. Together, these proteins transiently inhibit RNA polymerase II progression downstream of transcription start sites, enabling proper promoter-proximal pausing, RNA capping, and regulated gene expression.

NELFA interacts with DSIF (DRB sensitivity-inducing factor) and phosphorylated RNA polymerase II to modulate transcriptional timing and elongation efficiency. Release from pausing is mediated by P-TEFb-dependent phosphorylation events that allow productive elongation. Through these mechanisms, NELFA coordinates transcriptional responses to developmental, environmental, and signaling cues.

The NELFA antibody is widely used in transcription, chromatin, and epigenetic regulation research to study RNA polymerase II pausing, elongation control, and transcriptional activation. Western blot analysis identifies a 66 kilodalton band corresponding to NELFA, while immunofluorescence reveals nuclear punctate staining associated with active transcription foci. This antibody supports studies investigating transcriptional regulation and pause-release mechanisms.

Altered NELFA activity affects global gene expression programs, contributing to developmental disorders, cancer, and viral replication control. NELFA has also been linked to stress responses, where it modulates transcription restart following DNA damage or heat shock. The NELFA antibody provides a critical reagent for dissecting the molecular mechanisms of transcription elongation and RNA polymerase II regulation. NSJ Bioreagents offers this antibody validated for its applications, ensuring dependable performance in transcriptional research.

UniProt: Q9H3P2

Pathways: Chromatin Binding