SPO11 antibody (AA 40-326)

Catalog No. ABIN7874696

This Rabbit Polyclonal antibody specifically detects SPO11 in WB, ELISA, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SPO11 antibody (AA 40-326) (ABIN7874696)

Target: SPO11 (SPO11 Meiotic Protein Covalently Bound To DSB Homolog (SPO11))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPO11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SPO11 Antibody

Binding Specificity: AA 40-326

Purpose: SPO11 Antibody / Meiotic recombination protein SPO11

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SPO11 recombinant protein (Position: R40-D326) was used as the immunogen for the SPO11 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SPO11 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SPO11 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SPO11

Target: SPO11 (SPO11 Meiotic Protein Covalently Bound To DSB Homolog (SPO11))

Alternative Name: SPO11

Background: SPO11 antibody detects Meiotic recombination protein SPO11, encoded by the SPO11 gene. Meiotic recombination protein SPO11 is a topoisomerase-like enzyme that catalyzes the formation of programmed double-strand breaks during meiosis. SPO11 antibody provides researchers with a specific reagent for studying meiotic recombination, chromosome segregation, and fertility.

Meiotic recombination protein SPO11 is structurally related to archaeal topoisomerase VI subunits and uses a conserved tyrosine residue to generate covalent intermediates with DNA. Research using SPO11 antibody has shown that these breaks initiate homologous recombination, ensuring accurate pairing and exchange between homologous chromosomes. This process increases genetic diversity and is critical for gamete production.

Studies with SPO11 antibody have demonstrated that SPO11 activity is tightly regulated by accessory proteins, including REC114, MEI4, and others, which control timing and location of double-strand break formation. Without SPO11, homologous recombination fails, leading to meiotic arrest and infertility. These findings highlight its essential role in reproductive biology.

Dysfunction of Meiotic recombination protein SPO11 has been linked to infertility and meiotic disorders. Research using SPO11 antibody has shown that mutations impair break formation, resulting in abnormal gametogenesis. In model organisms, deletion of SPO11 causes sterility, while hypomorphic alleles produce meiotic defects. These observations emphasize its indispensable role in meiotic recombination.

SPO11 antibody is commonly used in western blotting, immunohistochemistry, and chromatin immunoprecipitation. Western blotting quantifies SPO11 expression in testis and ovary, immunohistochemistry reveals localization in meiotic cells, and chromatin immunoprecipitation identifies DNA binding and recombination sites. These applications make SPO11 antibody indispensable for reproductive biology research.

By providing validated SPO11 antibody reagents, NSJ Bioreagents supports studies into meiosis, recombination, and fertility. Detection of Meiotic recombination protein SPO11 provides researchers with insights into how double-strand breaks initiate homologous recombination and ensure chromosome segregation.

UniProt: Q9Y5K1