Recombinant Perforin 1 antibody (AA 413-552)

Catalog No. ABIN7874871

The Rabbit Monoclonal anti-Perforin 1 antibody is suitable to detect Perforin 1 in samples from Human. It has been validated for IHC (p).

Quick Overview for Recombinant Perforin 1 antibody (AA 413-552) (ABIN7874871)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Perforin 1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PRF1-7077R

Product Details anti-Perforin 1 Antibody

Binding Specificity: AA 413-552

Purpose: Perforin Antibody / PRF1

Purification: Protein A/G affinity

Immunogen: A portion of amino acids 413-552 was used as the immunogen for the PRF1 antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the PRF1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PRF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: Perforin

Background: Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.

UniProt: P14222

Pathways: Apoptosis, Caspase Cascade in Apoptosis