Perforin 1 antibody (AA 413-552)

Catalog No. ABIN7874873

This Mouse Monoclonal antibody specifically detects Perforin 1 in ELISA. It exhibits reactivity toward Human.

Quick Overview for Perforin 1 antibody (AA 413-552) (ABIN7874873)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Perforin 1 antibody is un-conjugated

Application: ELISA

Clone: PRF1-2467

Product Details anti-Perforin 1 Antibody

Binding Specificity: AA 413-552

Purpose: Perforin-1 Antibody / PRF1

Purification: Protein G affinity chromatography

Immunogen: A recombinant human partial protein (amino acids 413-552) was used as the immunogen for this Perforin-1 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the Perforin-1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Perforin-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: Perforin-1

Background: Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.

UniProt: P14222

Pathways: Apoptosis, Caspase Cascade in Apoptosis