Perforin 1 antibody (AA 413-552)

Catalog No. ABIN7874876

This Mouse Monoclonal antibody specifically detects Perforin 1 in ELISA. It exhibits reactivity toward Human.

Quick Overview for Perforin 1 antibody (AA 413-552) (ABIN7874876)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Perforin 1 antibody is un-conjugated

Application: ELISA

Grade: Carrier-free

Clone: PRF1-2468

Product Details anti-Perforin 1 Antibody

Binding Specificity: AA 413-552

Purpose: Perforin Antibody / PRF1 (azide and preservative free)

Purification: Protein G affinity

Immunogen: A portion of amino acids 413-552 from the human protein was used as the immunogen for the Perforin antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the Perforin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Perforin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: Perforin

Background: Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.

UniProt: P14222

Pathways: Apoptosis, Caspase Cascade in Apoptosis