TYRO3 antibody (AA 48-882)

Catalog No. ABIN7875218

This Rabbit Polyclonal antibody specifically detects TYRO3 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TYRO3 antibody (AA 48-882) (ABIN7875218)

Target: TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TYRO3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TYRO3 Antibody

Binding Specificity: AA 48-882

Purpose: TYRO3 Antibody / TYRO3 protein tyrosine kinase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TYRO3 recombinant protein (Position: A48-Q882) was used as the immunogen for the TYRO3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TYRO3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TYRO3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TYRO3

Target: TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))

Alternative Name: TYRO3

Background: TYRO3 antibody detects TYRO3 protein tyrosine kinase, encoded by the TYRO3 gene on chromosome 15q15.1. TYRO3 antibody is widely applied in studies of receptor tyrosine kinase signaling, cell survival, and immune regulation. TYRO3 is part of the TAM receptor family, which also includes AXL and MERTK. It functions as a receptor for ligands such as Gas6 and Protein S, activating intracellular signaling pathways that regulate cell proliferation, survival, phagocytosis, and immune modulation. Expression is broad, with high levels in brain, reproductive tissues, and immune cells.

Structurally, TYRO3 is a type I transmembrane protein with extracellular immunoglobulin-like domains that mediate ligand binding, followed by fibronectin type III domains, a single transmembrane helix, and an intracellular tyrosine kinase domain. Upon ligand binding, TYRO3 undergoes autophosphorylation and recruits signaling molecules including PI3K, GRB2, and SRC family kinases. These interactions activate pathways such as PI3K-AKT and MAPK, promoting survival and proliferation.

Functionally, TYRO3 contributes to tissue homeostasis by supporting cell survival, clearance of apoptotic cells, and regulation of immune responses. In neurons, it promotes survival and myelination. In immune cells, TYRO3 suppresses excessive inflammatory responses, maintaining tolerance and preventing autoimmunity. In reproductive biology, it supports spermatogenesis and fertility. Knockout models show neurological defects, immune dysregulation, and reproductive impairment. Researchers use TYRO3 antibody to study tyrosine kinase signaling, neural survival, and immune regulation.

Clinically, TYRO3 dysregulation is linked to cancer, autoimmune disease, and neurodegeneration. Overexpression of TYRO3 supports survival and drug resistance in cancers such as melanoma, glioblastoma, and breast cancer. In contrast, reduced TYRO3 signaling contributes to multiple sclerosis and lupus, where clearance of apoptotic cells is impaired. TYRO3 also has neuroprotective roles, with reduced activity associated with Parkinson's and Alzheimer's disease. NSJ Bioreagents offers TYRO3 antibody as a validated reagent for oncology, neurobiology, and immunology research.

Experimentally, TYRO3 antibody is applied in western blotting to detect the ~140 kDa protein, in immunohistochemistry to assess expression in tumors or brain tissue, and in immunofluorescence microscopy to study receptor localization. Immunoprecipitation with TYRO3 antibody reveals signaling complexes with ligands and kinases, providing insight into downstream pathways.

UniProt: Q06418

Pathways: RTK Signaling