PPP1R12A antibody (pThr696)

Catalog No. ABIN744068

The Rabbit Polyclonal anti-PPP1R12A antibody has been validated for WB and FACS. It is suitable to detect PPP1R12A in samples from Human and Rat.

Quick Overview for PPP1R12A antibody (pThr696) (ABIN744068)

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP1R12A antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-PPP1R12A Antibody

Binding Specificity: pThr696

Specificity: This phosphorylation site is homologous to that of Thr694 in Mouse and Thr697 in Rat. The region containing this phosphorylation site is known to be homologous to that of the related protein, PP12C, and will react with this protein when phosphorylated at Thr560 in Human and Mouse, and Thr561 in Rat.

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human around the phosphorylation site of Ser696

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
FCM 1:20-100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PPP1R12A

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Alternative Name: MYPT1

Background:

Synonyms: MBS, M130, MYPT1, Protein phosphatase 1 regulatory subunit 12A, Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit, PPP1R12A

Background: Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.

Gene ID: 4659

UniProt: O14974

Pathways: M Phase