SPHK1 antibody (AA 55-357)

Catalog No. ABIN7875687

The Rabbit Polyclonal anti-SPHK1 antibody is suitable to detect SPHK1 in samples from Human. It has been validated for WB, ELISA and FACS.

Quick Overview for SPHK1 antibody (AA 55-357) (ABIN7875687)

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPHK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SPHK1 Antibody

Binding Specificity: AA 55-357

Purpose: Sphingosine kinase 1 Antibody / SPHK1

Purification: Antigen affinity purified

Immunogen: E. coli-derived recombinant human protein (amino acids E55-N357) was used as the immunogen for the Sphingosine kinase 1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Sphingosine kinase 1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Sphingosine kinase 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SPHK1

Target: SPHK1 (Sphingosine Kinase 1 (SPHK1))

Alternative Name: Sphingosine kinase 1

Background: SPHK1 (Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.

UniProt: Q9NYA1

Pathways: VEGF Signaling