alpha Sarcomeric Actin (AA 557-692) antibody

Catalog No. ABIN7875750

The Mouse Monoclonal anti-alpha Sarcomeric Actin antibody is suitable to detect alpha Sarcomeric Actin in samples from Human. It has been validated for IHC (p) and WB.

Quick Overview for alpha Sarcomeric Actin (AA 557-692) antibody (ABIN7875750)

Target: alpha Sarcomeric Actin

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Grade: Carrier-free

Clone: ACTN2-3292

Product Details

Binding Specificity: AA 557-692

Purpose: Sarcomeric Alpha Actinin Antibody / ACTN2 (azide and preservative free)

Purification: Protein G affinity chromatography

Immunogen: Amino acids 557-692 from the human protein were used as the immunogen for this Sarcomeric Alpha Actinin antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: The optimal dilution of the Sarcomeric Alpha Actinin antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Sarcomeric Alpha Actinin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details

Target: alpha Sarcomeric Actin

Alternative Name: Sarcomeric Alpha Actinin

Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.

UniProt: P35609