RYK antibody (AA 66-607)

Catalog No. ABIN7876346

This Rabbit Polyclonal antibody specifically detects RYK in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RYK antibody (AA 66-607) (ABIN7876346)

Target: RYK (RYK Receptor-Like Tyrosine Kinase (RYK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RYK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RYK Antibody

Binding Specificity: AA 66-607

Purpose: RYK Antibody / Tyrosine-protein kinase RYK

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human RYK recombinant protein (Position: L66-V607) was used as the immunogen for the RYK antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RYK antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RYK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RYK

Target: RYK (RYK Receptor-Like Tyrosine Kinase (RYK))

Alternative Name: RYK

Background: RYK antibody detects Tyrosine-protein kinase RYK, an atypical receptor tyrosine kinase that mediates Wnt signaling during neural development and tissue morphogenesis. The UniProt recommended name is Tyrosine-protein kinase RYK (RYK). Unlike conventional kinases, RYK possesses a catalytically inactive kinase domain but functions as a receptor and co-receptor for Wnt family ligands, influencing cellular polarity and differentiation.

Functionally, RYK antibody identifies a 604-amino-acid single-pass transmembrane receptor that binds Wnt proteins through its extracellular WIF (Wnt inhibitory factor) domain. Upon ligand binding, RYK cooperates with Frizzled receptors to activate non-canonical Wnt signaling pathways such as planar cell polarity and calcium flux regulation. This signaling guides axon pathfinding, neuronal connectivity, and tissue patterning during embryogenesis.

The RYK gene is located on chromosome 3q22.1 and encodes a receptor expressed in developing brain, spinal cord, and epithelial tissues. Although structurally related to receptor tyrosine kinases, its kinase domain lacks catalytic residues, functioning instead as a scaffolding site for adaptor proteins and kinases that propagate downstream signals. RYK influences Wnt-dependent activation of Dishevelled, JNK, and Rho family GTPases, affecting cytoskeletal organization and migration.

In the nervous system, RYK is essential for axonal guidance, dendritic growth, and synaptic remodeling. Loss of RYK function disrupts neuronal wiring and corticospinal tract formation. In epithelial tissues, RYK contributes to planar cell polarity and branching morphogenesis. Aberrant RYK signaling has been associated with cancer, fibrosis, and neurodevelopmental disorders. Overexpression of RYK in tumors promotes invasive growth by modulating cell adhesion and Wnt-dependent transcriptional programs.

RYK antibody is widely used in developmental biology, neurobiology, and signaling research. It is suitable for immunoblotting, immunofluorescence, and receptor binding studies to detect RYK expression and localization at the plasma membrane. In neuroscience, this antibody supports studies of Wnt receptor composition and axon guidance. In cancer research, RYK detection helps characterize Wnt-driven signaling and invasive phenotypes.

Structurally, RYK features an extracellular WIF domain that binds Wnt ligands, a single transmembrane helix, and an intracellular pseudokinase domain that serves as a docking platform for signaling complexes. Its signaling capacity depends on proteolytic cleavage and release of the intracellular domain, which can translocate to the nucleus and modulate gene transcription. NSJ Bioreagents provides RYK antibody reagents validated for use in Wnt signaling, receptor biology, and neurodevelopmental research.

UniProt: P34925

Pathways: RTK Signaling, WNT Signaling, Regulation of Cell Size