CDCA8 antibody (AA 72-280)

Catalog No. ABIN7876633

The Rabbit Polyclonal anti-CDCA8 antibody (ABIN7876633) specifically detects CDCA8 in WB, ELISA, FACS, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for CDCA8 antibody (AA 72-280) (ABIN7876633)

Target: CDCA8 (Cell Division Cycle Associated 8 (CDCA8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDCA8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CDCA8 Antibody

Binding Specificity: AA 72-280

Purpose: CDCA8 Antibody / Cell division cycle-associated protein 8 / Borealin

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human CDCA8 recombinant protein (Position: D72-K280) was used as the immunogen for the CDCA8 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CDCA8 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CDCA8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for CDCA8

Target: CDCA8 (Cell Division Cycle Associated 8 (CDCA8))

Alternative Name: CDCA8

Background: CDCA8 antibody recognizes Cell division cycle-associated protein 8, also known as Borealin, a core component of the chromosomal passenger complex (CPC) that governs chromosome segregation, spindle assembly, and cytokinesis. Encoded by the CDCA8 gene on chromosome 1p34.3, this protein interacts with Aurora B kinase, INCENP, and Survivin to form a functional complex that ensures accurate mitotic progression. Borealin is essential for proper kinetochore-microtubule attachment, spindle checkpoint activation, and correction of mitotic errors, thereby safeguarding genomic stability during cell division.

During mitosis, Borealin localizes to centromeres in metaphase and relocates to the spindle midzone during anaphase, coordinating the transition from chromosome alignment to cytokinesis. Loss of CDCA8 function results in chromosome missegregation, lagging chromatids, and multinucleation, phenotypes commonly associated with aneuploidy and cancer. Expression of CDCA8 peaks during the G2/M phase, reflecting its cell cycle-dependent regulation. Overexpression has been documented in a range of malignancies including breast, lung, and colorectal cancers, correlating with poor prognosis and high proliferative indices.

The CDCA8 antibody is widely used in cell cycle and cancer research to detect Borealin localization and expression dynamics. Immunofluorescence analysis with this antibody reveals distinct centromeric staining during metaphase and midbody localization in late mitosis. Western blotting identifies a 35 kilodalton band corresponding to the full-length protein. Because the chromosomal passenger complex regulates essential mitotic checkpoints, the CDCA8 antibody provides a critical tool for studying mechanisms of mitotic fidelity and tumorigenesis.

CDCA8 cooperates with Aurora B to phosphorylate substrates involved in spindle tension sensing and cytokinesis. Disruption of this interaction impairs CPC activity and leads to mitotic failure. Borealin's ability to bind DNA through its N-terminal domain and oligomerize via its coiled-coil region enables structural stability of the CPC. Inhibition or knockdown of CDCA8 sensitizes tumor cells to microtubule-targeting drugs, highlighting its potential as a therapeutic target. The antibody thus supports translational research exploring CPC inhibitors and mitotic checkpoint modulators in cancer therapy.

In developmental biology, CDCA8 is required for proper cell division during embryogenesis and organogenesis. The CDCA8 antibody facilitates identification of proliferating cells in rapidly dividing tissues and embryonic stem cell cultures. NSJ Bioreagents provides this antibody validated for its applications, ensuring consistent results in mitotic and oncogenic studies.

UniProt: Q53HL2

Pathways: Cell Division Cycle, M Phase