RBX1 antibody (AA 78-108)

Catalog No. ABIN7876777

The Rabbit Polyclonal anti-RBX1 antibody (ABIN7876777) specifically detects RBX1 in WB, IF and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for RBX1 antibody (AA 78-108) (ABIN7876777)

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RBX1 Antibody

Binding Specificity: AA 78-108

Purpose: RBX1 Antibody

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 78-108 from the human protein were used as the immunogen for the RBX1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the RBX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the RBX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for RBX1

Target: RBX1 (Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1))

Alternative Name: RBX1

Background: E3 ubiquitin ligase component of multiple cullin-RING- based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.

UniProt: P62877

Pathways: Cell Division Cycle, M Phase, SARS-CoV-2 Protein Interactome