MIB1 antibody (AA 78-995)

Catalog No. ABIN7876788

The Rabbit Polyclonal anti-MIB1 antibody is suitable to detect MIB1 in samples from Zebrafish (Danio rerio). It has been validated for WB.

Quick Overview for MIB1 antibody (AA 78-995) (ABIN7876788)

Target: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIB1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MIB1 Antibody

Binding Specificity: AA 78-995

Purpose: Zebrafish Mib1 Antibody / Mind bomb 1

Purification: Antigen affinity chromatography

Immunogen: E. coli-derived zebrafish Mib1 recombinant protein (amino acids I78-K995) was used as the immunogen for the Zebrafish Mib1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Mib1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Mib1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MIB1

Target: MIB1 (Mindbomb E3 Ubiquitin Protein Ligase 1 (MIB1))

Alternative Name: Mib1

Background: Mib1, or mind bomb one, is an essential E three ubiquitin ligase that plays a critical role in the regulation of Notch signaling during vertebrate development. In zebrafish, Mib1 is necessary for the proper functioning of the Notch pathway, which controls cell fate decisions, tissue patterning, and the formation of organs such as the brain, spinal cord, and somites.

Mib1 functions by promoting the ubiquitination and endocytosis of Delta ligands on the surface of signal sending cells. This endocytosis is required for activation of the Notch receptor in neighboring cells. In zebrafish embryos, Mib1 is expressed in regions undergoing active cell differentiation and tissue segmentation. Loss of Mib1 function leads to widespread defects in neurogenesis, somitogenesis, and other Notch dependent processes.

Studies using zebrafish mutants have shown that Mib1 is critical for lateral inhibition in the developing nervous system, the formation of the segmented body axis, and the maintenance of progenitor pools in various tissues. Because of its conserved function across vertebrates, zebrafish Mib1 is a widely used model for understanding the molecular mechanisms of Notch signaling and its role in development and disease.

Zebrafish Mib1 is especially valuable in genetic and pharmacological screens aimed at identifying modifiers of the Notch pathway and related developmental disorders.

UniProt: Q804S5

Pathways: SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection