MDM4-binding Protein antibody (AA 8-490)

Catalog No. ABIN7876836

The Rabbit Polyclonal anti-MDM4-binding Protein antibody is suitable to detect MDM4-binding Protein in samples from Human. It has been validated for WB and ELISA.

Quick Overview for MDM4-binding Protein antibody (AA 8-490) (ABIN7876836)

Target: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MDM4-binding Protein antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MDM4-binding Protein Antibody

Binding Specificity: AA 8-490

Purpose: MDMX Antibody / MDM4

Purification: Antigen affinity purified

Immunogen: An E.coli-derived human recombinant protein (A8-A490) was used as the immunogen for the MDMX antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MDMX antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MDMX antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MDM4-binding Protein

Target: MDM4-binding Protein (MDM4) (Mdm4-binding Protein (MDM4))

Alternative Name: MDMX

Background: Protein Mdm4/Mdmx is a protein that in humans is encoded by the MDM4 gene. This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

UniProt: O15151

Pathways: Cell Division Cycle