PDK3 antibody (AA 84-406)

Catalog No. ABIN7876958

This Rabbit Polyclonal antibody specifically detects PDK3 in WB, ELISA, FACS, IHC (p) and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PDK3 antibody (AA 84-406) (ABIN7876958)

Target: PDK3 (Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-PDK3 Antibody

Binding Specificity: AA 84-406

Purpose: PDK3 Antibody / Pyruvate dehydrogenase kinase 3

Purification: Antigen affinity purified

Immunogen: An E.coli-derived human recombinant protein (amino acids Y84-Q406) was used as the immunogen for the PDK3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PDK3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PDK3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PDK3

Target: PDK3 (Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3))

Alternative Name: PDK3

Background: Pyruvate dehydrogenase lipoamide kinase isozyme 3, mitochondrial is an enzyme that in humans is encoded by the PDK3 gene. The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt: Q15120

Pathways: PI3K-Akt Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect