NUP88 antibody (AA 91-584)

Catalog No. ABIN7877141

This Rabbit Polyclonal antibody specifically detects NUP88 in WB, ICC, ELISA and IF. It exhibits reactivity toward Human.

Quick Overview for NUP88 antibody (AA 91-584) (ABIN7877141)

Target: NUP88 (Nucleoporin 88kDa (NUP88))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUP88 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA, Immunofluorescence (IF)

Product Details anti-NUP88 Antibody

Binding Specificity: AA 91-584

Purpose: NUP88 Antibody / Nuclear pore complex protein Nup88 / Nucleoporin 88

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human NUP88 recombinant protein (Position: E91-K584) was used as the immunogen for the NUP88 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NUP88 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NUP88 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NUP88

Target: NUP88 (Nucleoporin 88kDa (NUP88))

Alternative Name: NUP88

Background: NUP88 antibody detects Nuclear pore complex protein Nup88, a structural component of the nuclear pore complex that regulates nucleocytoplasmic transport. The UniProt recommended name is Nuclear pore complex protein Nup88 (NUP88). This protein interacts with other nucleoporins to facilitate the bidirectional exchange of macromolecules between the nucleus and cytoplasm, maintaining cellular communication and homeostasis.

Functionally, NUP88 antibody identifies a 741-amino-acid nucleoporin localized to the cytoplasmic face of the nuclear pore complex. NUP88 forms a subcomplex with NUP214 and NUP62 that assists in nuclear export of ribonucleoproteins and protein cargoes. It is also involved in spindle assembly and chromosome segregation during mitosis, highlighting its dual role in transport and cell cycle control.

The NUP88 gene is located on chromosome 17p13.1 and is ubiquitously expressed in proliferating cells. Its expression is dynamically regulated during mitosis and stress responses. NUP88i 1/2s proper function ensures balanced nucleocytoplasmic transport and mitotic progression, while its mislocalization disrupts nuclear integrity.

Pathologically, overexpression of NUP88 has been linked to several cancers, including breast, colorectal, and hepatocellular carcinoma, where it promotes nuclear transport of oncogenic factors. Mutations in NUP88 cause neuromuscular and developmental disorders such as fetal akinesia deformation sequence. Research using NUP88 antibody supports studies in nuclear transport, mitosis, and tumor biology.

NUP88 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect nuclear pore complex proteins. NSJ Bioreagents provides NUP88 antibody reagents optimized for studies in cell biology, nuclear transport, and cancer research.

Structurally, Nuclear pore complex protein Nup88 contains coiled-coil domains that mediate interaction with other nucleoporins and a C-terminal alpha-helical region that anchors it within the nuclear envelope. This antibody aids in analyzing NUP88's role in nuclear transport and mitotic regulation.

UniProt: Q99567

Pathways: SARS-CoV-2 Protein Interactome