Recombinant alpha Tubulin antibody (acLys40)

Catalog No. ABIN7877304

The Rabbit Monoclonal anti-alpha Tubulin antibody is suitable to detect alpha Tubulin in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF, ICC, IP and FACS.

Quick Overview for Recombinant alpha Tubulin antibody (acLys40) (ABIN7877304)

Target: alpha Tubulin (TUBA1)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This alpha Tubulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: 31T79

Product Details anti-alpha Tubulin Antibody

Binding Specificity: acLys40

Purpose: Alpha Tubulin (acetyl K40) Antibody / TUBA1B/TUBA4A

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human alpha Tubulin (acetyl K40) was used as the immunogen for the Alpha Tubulin (acetyl K40) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Alpha Tubulin (acetyl K40) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Alpha Tubulin (acetyl K40) antibody at -20oC.

Target Details for alpha Tubulin

Target: alpha Tubulin (TUBA1)

Alternative Name: Alpha Tubulin

Background: Alpha Tubulin (acetyl K40) antibody detects alpha tubulin specifically when acetylated at lysine 40. Alpha tubulin, encoded by multiple genes including TUBA1A, TUBA3C, and TUBA4A, is one of the two principal subunits of microtubules, the dynamic cytoskeletal polymers critical for cell shape, intracellular transport, and mitotic spindle formation. Common alternate identifiers include TUBA1 and TUBA4, which are widely referenced in literature. Acetylation of lysine 40 is a well known post translational modification associated with stable, long lived microtubules that support transport and signaling.

Alpha Tubulin (acetyl K40) antibody is widely applied in cell biology, neurobiology, and cancer research. Microtubule acetylation is enriched in neuronal axons, cilia, and stable cytoskeletal structures. By detecting acetylation at lysine 40, researchers can evaluate how microtubules achieve stability and how post translational modifications regulate cytoskeletal dynamics.

Immunofluorescence with Alpha Tubulin (acetyl K40) antibody highlights stable microtubule tracks in neurons and ciliated cells. Immunohistochemistry maps tissue regions enriched in acetylated tubulin, while western blotting detects the modified isoform as distinct from total tubulin. These assays provide powerful tools for studying cytoskeletal regulation.

Acetylation at lysine 40 is mediated by alpha tubulin acetyltransferase (ATAT1) and reversed by histone deacetylase 6. Dysregulation of this balance contributes to neurodegenerative disease, cancer, and ciliopathies. By applying this phospho specific antibody, scientists can examine mechanisms of axonal transport, sensory signaling, and tumor progression.

Alpha Tubulin (acetyl K40) antibody from NSJ Bioreagents provides strong specificity for detecting this critical post translational modification. Its performance supports both basic and translational studies of microtubule biology.

UniProt: P68363

Pathways: Microtubule Dynamics