GPX3 antibody (C-Term)

Catalog No. ABIN7877385

The Rabbit Polyclonal anti-GPX3 antibody is suitable to detect GPX3 in samples from Mouse and Rat. It has been validated for WB.

Quick Overview for GPX3 antibody (C-Term) (ABIN7877385)

Target: GPX3 (Glutathione Peroxidase 3 (Plasma) (GPX3))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPX3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GPX3 Antibody

Binding Specificity: C-Term

Purpose: GPX3 Antibody / Glutathione peroxidase 3

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GPX3 was used as the immunogen for the GPX3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GPX3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GPX3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GPX3

Target: GPX3 (Glutathione Peroxidase 3 (Plasma) (GPX3))

Alternative Name: GPX3

Background: GPX3 antibody detects Glutathione peroxidase 3, a secreted antioxidant enzyme that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides using glutathione. The UniProt recommended name is Glutathione peroxidase 3 (GPX3). This selenoprotein plays a key role in extracellular redox homeostasis and protection against oxidative damage in plasma and other biological fluids.

Functionally, GPX3 antibody identifies a 226-amino-acid glycoprotein containing a selenocysteine residue at its active site. GPX3 is primarily synthesized in the kidney proximal tubules and secreted into the plasma, where it scavenges peroxides to prevent oxidative injury to lipids, proteins, and DNA. Its catalytic activity depends on the presence of glutathione as a reducing cofactor and selenium availability for selenocysteine biosynthesis.

The GPX3 gene is located on chromosome 5q33.1 and encodes the only extracellular form among the glutathione peroxidase family. It is expressed in kidney, lung, liver, and several endocrine tissues, contributing to systemic antioxidant defense. GPX3 levels fluctuate with oxidative stress, inflammation, and selenium intake, making it a biomarker of redox status.

Pathologically, reduced GPX3 expression has been linked to cancer, diabetes, and cardiovascular diseases. Loss of GPX3 activity enhances oxidative DNA damage and supports tumor growth through redox imbalance. Conversely, elevated GPX3 expression in response to stress reflects a protective adaptation. Research with GPX3 antibody aids studies in oxidative stress, redox biology, and disease biomarker development.

GPX3 antibody is suitable for western blotting, immunohistochemistry, and ELISA to measure GPX3 protein in serum, plasma, and tissues. NSJ Bioreagents provides validated GPX3 antibody reagents optimized for oxidative stress, metabolism, and antioxidant pathway research.

Structurally, Glutathione peroxidase 3 forms a homotetramer with each subunit containing a conserved catalytic triad for peroxide reduction. The enzymei 1/2s secretion signal sequence directs it to the extracellular space, where it complements intracellular antioxidant systems. This antibody enables quantitative and qualitative analysis of GPX3 in health and disease contexts.

UniProt: P22352

Pathways: Thyroid Hormone Synthesis