AMACR antibody (full length)

Catalog No. ABIN7877645

This Rabbit Monoclonal antibody specifically detects AMACR in WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for AMACR antibody (full length) (ABIN7877645)

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This AMACR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 13H4

Product Details anti-AMACR Antibody

Binding Specificity: full length

Purpose: p504S Antibody / AMACR

Purification: Protein A affinity chromatography

Immunogen: Full length human recombinant protein was used as the immunogen for the p504S antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the p504S antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the p504S antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for AMACR

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Alternative Name: p504S

Background: This antibody recognizes a protein of 44 kDa, which is identified as AMACR, also known as p504S. It is an enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids. AMACR is essential in lipid metabolism. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands. AMACR is expressed in normal liver (hepatocytes), kidney (tubular epithelial cells) and gall bladder (epithelial cells). Expression has also been found in lung (bronchial epithelial cells) and colon (colonic surface epithelium). AMACR expression can also be found in hepatocellular carcinoma and kidney carcinoma. Past studies have also shown that AMACR is expressed in various colon carcinomas (well, moderately and poorly differentiated) and over expressed in prostate carcinoma.

UniProt: Q9UHK6

Pathways: Monocarboxylic Acid Catabolic Process