Cyclin B2 antibody (full length)

Catalog No. ABIN7878065

This Mouse Monoclonal antibody specifically detects Cyclin B2 in ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Cyclin B2 antibody (full length) (ABIN7878065)

Target: Cyclin B2 (CCNB2)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B2 antibody is un-conjugated

Application: ELISA

Clone: X29-2

Product Details anti-Cyclin B2 Antibody

Binding Specificity: full length

Purpose: Cyclin B2 Antibody / CCNB2

Purification: Protein G affinity chromatography

Immunogen: Recombinant full-length Xenopus laevis Cyclin B2 protein was used as the immunogen for this Cyclin B2 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Cyclin B2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Cyclin B2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Cyclin B2

Target: Cyclin B2 (CCNB2)

Alternative Name: Cyclin B2

Background: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.

UniProt: O95067

Pathways: Cell Division Cycle, M Phase