LIG1 antibody (full length)

Catalog No. ABIN7878535

The Mouse Monoclonal anti-LIG1 antibody (Clone 1A9) (ABIN7878535) specifically detects LIG1 in WB and ELISA. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for LIG1 antibody (full length) (ABIN7878535)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 1A9

Product Details anti-LIG1 Antibody

Binding Specificity: full length

Purpose: DNA Ligase 1 Antibody / LIG1

Purification: Protein G affinity chromatography

Immunogen: Full-length native cow LIG1 protein was used as the immunogen for the DNA Ligase 1 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the DNA Ligase 1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the DNA Ligase 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Alternative Name: DNA Ligase 1

Background: DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. Furthermore, DNA Ligase I antisense ODNs syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme.

UniProt: P18858

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA