MED21 antibody (full length)

Catalog No. ABIN7878591

The Mouse Monoclonal anti-MED21 antibody is suitable to detect MED21 in samples from Human. It has been validated for WB, ELISA, IF and FACS.

Quick Overview for MED21 antibody (full length) (ABIN7878591)

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MED21 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-MED21-4B5

Product Details anti-MED21 Antibody

Binding Specificity: full length

Purpose: MED21 Antibody / SRB7

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human MED21 protein was used as the immunogen for the MED21 antibody.

Isotype: IgG1

Application Details

Application Notes: Optimal dilution of the MED21 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MED21 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for MED21

Target: MED21 (Mediator Complex Subunit 21 (MED21))

Alternative Name: MED21

Background: In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase. These complexes include the SMCC (SRB and MED protein cofactor complex), which consists of various subunits that share homology with several components of the yeast transcriptional mediator complexes and including the human proteins Srb7, Med6 (also designated DRIP33) and Med7 (also designated DRIP34). SMCC associates with the RNAPII (RNA polymerase II) holoenzyme through Srb7 and, in turn, enhances gene-specific activation or repression induced by DNA-binding transcription factors. Med6 and Med7, as well as other components of SMCC, associate with co-activator proteins from the TRAP (thyroid hormone receptor-activating protein) complex and DRIP (for vitamin D receptor interacting protein) complex to facilitate steroid receptor dependent transcriptional activation. Additionally, SMCC associates with PC4 (positive cofactor 4) to repress basal transcription independent of RNAPII activity

UniProt: Q13503

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha