MBD3 antibody (full length)

Catalog No. ABIN7878616

The Mouse Monoclonal anti-MBD3 antibody (Clone PCRP-MBD3-1C4) (ABIN7878616) specifically detects MBD3 in IF and FACS. The antibody is reactive with Human samples.

Quick Overview for MBD3 antibody (full length) (ABIN7878616)

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MBD3 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-MBD3-1C4

Product Details anti-MBD3 Antibody

Binding Specificity: full length

Purpose: MBD3 Antibody

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human MBD3 protein was used as the immunogen for the MBD3 antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the MBD3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MBD3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for MBD3

Target: MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))

Alternative Name: MBD3

Background: Methylation of DNA contributes to the regulation of gene transcription in both mammalian and invertebrate systems. DNA methylation predominates on cytosine residues that are present in dinucleotide motifs consisting of a 5 they include methyl-CpG binding protein 1 (MBD1), MBD2, MBD3, MBD4 and MeCP2. Expression of the MBD proteins is highest in somatic tissues. MBD1 binds in a context specific manner to methyl-CpG rich domains and, in turn, mediates the transcriptional inhibition that is commonly observed with DNA methylation. Similarly, MBD2 inhibits transcription of methylated genes by associating with histone deacetylase (HDAC1) within the MeCP1 repressor complex. In addition, MBD4, which is also designated MED1, associates with the mismatch repair protein MLH1 and preferentially binds to methylated cytosine residues in mismatched base pairs. MeCP2 binds tightly to chromosomes in a methylation-dependent manner and associates with a corepressor complex containing the transcriptional repressor mSin3A and histone deacetylases. MeCP2 binds tightly to chromosomes in a methylation-dependent manner and associates with a corepressor complex containing the transcriptional repressor mSin3A and histone deacetylases.

UniProt: O95983

Pathways: Chromatin Binding