MBNL3 antibody (full length)

Catalog No. ABIN7878660

The Mouse Monoclonal anti-MBNL3 antibody is suitable to detect MBNL3 in samples from Human. It has been validated for FACS.

Quick Overview for MBNL3 antibody (full length) (ABIN7878660)

Target: MBNL3 (Muscleblind-Like Splicing Regulator 3 (MBNL3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MBNL3 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: PCRP-MBNL3-1D11

Product Details anti-MBNL3 Antibody

Binding Specificity: full length

Purpose: MBNL3 Antibody

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human MBNL3 protein was used as the immunogen for the MBNL3 antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the MBNL3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MBNL3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for MBNL3

Target: MBNL3 (Muscleblind-Like Splicing Regulator 3 (MBNL3))

Alternative Name: MBNL3

Background: Pre-mRNA splicing is a critical step in the posttranscriptional regulation of gene expression. Several protein complexes are involved in proper mRNA splicing and transport. The muscleblind proteins, MBNL1, MBNL2 and MBNL3, promote inclusion or exclusion of specific exons on different pre-mRNAs by antagonizing the activity of CUG-BP and ETR-3-like factors bound to distinct intronic sites. MBNL1 and 2, which associate with expanded CUG repeats in vitro, localize to the nuclear foci in both DM1 and DM2 (myotonic dystrophy types 1 and 2), suggesting that the nuclear accumulation of mutant RNA is pathogenic in DM1, therefore implicating MBNL1 and 2 in the pathogenesis of both disorders. MBNL3, a 354 amino acid protein, inhibits expression of muscle differentiation, opposite to the function of MBNL1, which functions as a promoter of muscle differentiation. MBNL3 shows strong expression in placenta.

UniProt: Q9NUK0

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development