SP100 antibody (full length)

Catalog No. ABIN7879068

The Mouse Monoclonal anti-SP100 antibody is suitable to detect SP100 in samples from Human. It has been validated for WB, ELISA, IP, IF, FACS and IHC (p).

Quick Overview for SP100 antibody (full length) (ABIN7879068)

Target: SP100 (SP100 Nuclear Antigen (SP100))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SP100 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PCRP-SP100-1B9

Product Details anti-SP100 Antibody

Binding Specificity: full length

Purpose: SP100 Antibody

Purification: Protein A/G affinity

Immunogen: Recombinant full-length human SP100 protein was used as the immunogen for the SP100 antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the SP100 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the SP100 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for SP100

Target: SP100 (SP100 Nuclear Antigen (SP100))

Alternative Name: SP100

Background: The human SP100 gene encodes an autoantigen that co-localizes with PML and NDP52 in distinct nuclear domains, called nuclear dots (NDs) or ND10 nuclear bodies. Papova-, adeno-, and herpesviruses begin their transcription and DNA-replication at NDs, which play a role in autoimmunity, viral infections and in the etiology of acute promyelocytic leukemia. SP-100 is an interferon inducible protein that has two splice variants. One splice variant contains a highly conserved copy of the DNA-binding high mobility group 1 protein sequence, and thus represents a novel HMG-box protein. This alternatively spliced variant of SP-100 has a unique expression and localization pattern that is distinct from the SP-100 full-length protein. The SP100 protein is covalently modified by the small ubiquitin-related protein SUMO-1. SP-100 contains a functional nuclear localization signal and an ND-targeting region, which overlaps with the SP-100 homodimerization domain.

UniProt: P23497

Pathways: Retinoic Acid Receptor Signaling Pathway