Recombinant TOP1MT antibody (full length)

Catalog No. ABIN7879161

The Rabbit Monoclonal anti-TOP1MT antibody is suitable to detect TOP1MT in samples from Human. It has been validated for IHC (p).

Quick Overview for Recombinant TOP1MT antibody (full length) (ABIN7879161)

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TOP1MT antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TPIMT-2R

Product Details anti-TOP1MT Antibody

Binding Specificity: full length

Purpose: Recombinant TOP1MT Antibody

Purification: Protein A affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant TOP1MT antibody.

Isotype: IgG, kappa

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant TOP1MT antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant TOP1MT antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for TOP1MT

Target: TOP1MT (Topoisomerase (DNA) I, Mitochondrial (TOP1MT))

Alternative Name: TOP1MT

Background: DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

UniProt: Q969P6