PECR antibody (Middle Region)

Catalog No. ABIN7879538

This Rabbit Polyclonal antibody specifically detects PECR in WB, IHC and IF. It exhibits reactivity toward Human and Rat.

Quick Overview for PECR antibody (Middle Region) (ABIN7879538)

Target: PECR (Peroxisomal Trans-2-Enoyl-CoA Reductase (PECR))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PECR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PECR Antibody

Binding Specificity: Middle Region

Purpose: PECR Antibody / Peroxisomal trans-2-enoyl-CoA reductase

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PECR was used as the immunogen for the PECR antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PECR antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PECR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PECR

Target: PECR (Peroxisomal Trans-2-Enoyl-CoA Reductase (PECR))

Alternative Name: PECR

Background: PECR antibody detects Peroxisomal trans-2-enoyl-CoA reductase, an oxidoreductase enzyme that participates in the peroxisomal fatty acid beta-oxidation pathway. The UniProt recommended name is Peroxisomal trans-2-enoyl-CoA reductase (PECR). This enzyme catalyzes the NADPH-dependent reduction of trans-2-enoyl-CoA intermediates to acyl-CoA during the chain elongation and degradation of fatty acids, thereby maintaining lipid homeostasis and energy balance.

Functionally, PECR antibody identifies a 303-amino-acid peroxisomal matrix enzyme encoded by the PECR gene, which contributes to the reduction step of unsaturated fatty acid metabolism. PECR participates in both peroxisomal beta-oxidation and fatty acid elongation cycles, converting trans-2-enoyl-CoA substrates into saturated acyl-CoA derivatives for further processing. It plays a critical role in maintaining normal lipid composition within cellular membranes and is essential for the metabolism of very long-chain and polyunsaturated fatty acids.

The PECR gene is located on chromosome 2q35 and is expressed in liver, kidney, brain, and skeletal muscle. Its expression is regulated by peroxisome proliferator-activated receptors (PPARs), linking it to lipid metabolism and oxidative stress responses. The enzyme's localization to peroxisomes allows it to cooperate with other beta-oxidation enzymes such as ACOX1 and HSD17B4.

Pathologically, PECR dysfunction or mutation has been associated with metabolic abnormalities and neurodegenerative disease. Variants in PECR can lead to abnormal lipid accumulation and impaired energy metabolism, contributing to disorders such as Refsum-like disease and retinal dystrophy. Research using PECR antibody supports studies in lipid metabolism, peroxisomal function, and oxidative stress regulation.

PECR antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect peroxisomal enzymes. NSJ Bioreagents provides PECR antibody reagents optimized for studies in fatty acid oxidation, lipid homeostasis, and metabolic enzyme regulation.

Structurally, Peroxisomal trans-2-enoyl-CoA reductase belongs to the short-chain dehydrogenase/reductase (SDR) family and contains conserved catalytic motifs (TGxxxGxG and YxxxK) required for NADPH binding and substrate reduction. The enzyme forms homotetramers within peroxisomes and contributes to redox balance via NADPH utilization. This antibody allows investigation of PECR's role in peroxisomal metabolism, energy balance, and cellular redox homeostasis.

UniProt: Q9BY49