Annexin A2 antibody (N-Term)

Catalog No. ABIN7879560

The Rabbit Polyclonal anti-Annexin A2 antibody is suitable to detect Annexin A2 in samples from Human, Mouse and Rat. It has been validated for WB and FACS.

Quick Overview for Annexin A2 antibody (N-Term) (ABIN7879560)

Target: Annexin A2 (ANXA2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Annexin A2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-Annexin A2 Antibody

Binding Specificity: N-Term

Purpose: ANXA2 Antibody / Annexin A2

Purification: Immunogen affinity purified

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ANXA2 was used as the immunogen for the ANXA2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ANXA2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ANXA2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Annexin A2

Target: Annexin A2 (ANXA2)

Alternative Name: ANXA2

Background: ANXA2 antibody detects Annexin A2, a calcium-dependent phospholipid-binding protein involved in membrane organization, cytoskeletal interactions, and fibrinolysis. Encoded by the ANXA2 gene on chromosome 15q26.1, this multifunctional protein participates in membrane trafficking, endocytosis, cell-cell adhesion, and extracellular matrix remodeling. Annexin A2 also acts as a cell surface receptor for tissue plasminogen activator (tPA) and plasminogen, facilitating localized fibrin degradation and vascular repair.

Structurally, Annexin A2 is a 339-amino-acid protein of approximately 39 kilodaltons containing four annexin repeats that mediate calcium-dependent phospholipid binding and a unique N-terminal domain that interacts with S100A10 (p11) to form a heterotetrameric complex. This complex localizes to the plasma membrane, cytoskeleton, and endosomal compartments, where it modulates vesicle fusion, cytoskeletal anchoring, and membrane curvature. Annexin A2 is expressed in endothelial, epithelial, and tumor cells, as well as macrophages and neurons.

The ANXA2 antibody is widely used in cell biology, vascular biology, and cancer research to study membrane organization, signal transduction, and fibrinolytic regulation. Western blot analysis detects a 39 kilodalton band corresponding to Annexin A2, while immunofluorescence shows cytoplasmic, membrane, and perinuclear localization. This antibody provides a powerful reagent for investigating calcium-dependent membrane remodeling and extracellular proteolytic activity.

Functionally, Annexin A2 regulates actin cytoskeleton rearrangement and vesicle trafficking, influencing endocytosis, exocytosis, and cell motility. At the cell surface, it promotes plasmin generation, contributing to fibrin degradation, angiogenesis, and tumor invasion. Dysregulation of ANXA2 expression is associated with cancer progression, thrombosis, and inflammatory disease. The ANXA2 antibody supports detailed studies of its role in cellular dynamics, extracellular matrix remodeling, and vascular homeostasis. NSJ Bioreagents validates this antibody for its applications, ensuring precise detection in studies of cytoskeletal and membrane biology.

UniProt: P07355

Pathways: S100 Proteins