Recombinant MPRIP antibody (pSer232)

Catalog No. ABIN7879721

This Rabbit Monoclonal antibody specifically detects MPRIP in WB. It exhibits reactivity toward Mouse and Rat.

Quick Overview for Recombinant MPRIP antibody (pSer232) (ABIN7879721)

Target: MPRIP (Myosin Phosphatase rho Interacting Protein (MPRIP))

Antibody Type: Recombinant Antibody

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MPRIP antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 31R36

Product Details anti-MPRIP Antibody

Binding Specificity: pSer232

Purpose: Phospho-RIP3 (Ser232) Antibody / RIPK3

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human Phospho-RIP3 (S232) was used as the immunogen for the Phospho-RIP3 (Ser232) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-RIP3 (Ser232) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-RIP3 (Ser232) antibody at -20oC.

Target Details for MPRIP

Target: MPRIP (Myosin Phosphatase rho Interacting Protein (MPRIP))

Alternative Name: RIP3

Background: Phospho-RIP3 (Ser232) antibody detects RIP3 phosphorylated at serine 232, encoded by the RIPK3 gene. RIP3, also called Receptor interacting serine threonine kinase 3, is a critical mediator of necroptosis, a regulated form of necrotic cell death. Phospho-RIP3 (Ser232) antibody provides researchers with a specific reagent to study necroptotic signaling, inflammation, and disease mechanisms involving programmed necrosis.

RIP3 functions as a core signaling kinase within the necrosome complex. Research using Phospho-RIP3 (Ser232) antibody has shown that phosphorylation at Ser232 is essential for RIP3 activation and subsequent recruitment of MLKL, the executioner protein of necroptosis. This phosphorylation serves as a molecular switch controlling progression of the necroptotic pathway.

Studies with Phospho-RIP3 (Ser232) antibody have revealed that necroptosis is triggered by tumor necrosis factor signaling, viral infection, and cellular stress conditions. RIP3 phosphorylation at Ser232 enables necrosome assembly, MLKL oligomerization, and disruption of plasma membrane integrity. This process releases intracellular contents that amplify inflammation and immune responses.

In disease contexts, dysregulated necroptosis contributes to pathology. Research using Phospho-RIP3 (Ser232) antibody has shown that excessive activation promotes tissue damage in conditions such as ischemia reperfusion injury, inflammatory bowel disease, and neurodegeneration. Conversely, insufficient necroptosis facilitates viral persistence and impaired immune clearance. These findings highlight RIP3 phosphorylation as a therapeutic target for modulating necroptotic signaling.

Phospho-RIP3 (Ser232) antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting distinguishes phosphorylated RIP3 from total levels, immunohistochemistry localizes activated necroptotic signaling in tissue, and immunofluorescence reveals subcellular distribution during necrosome formation. These applications make the antibody valuable in cell death research.

By providing validated Phospho-RIP3 (Ser232) antibody reagents, NSJ Bioreagents supports studies into programmed necrosis, inflammation, and therapeutic modulation. Detection of RIP3 phosphorylated at Ser232 provides insight into the molecular regulation of necroptosis and its contribution to disease.

UniProt: Q9Y572