Recombinant UBTF antibody (pSer484)

Catalog No. ABIN7879748

This Rabbit Monoclonal antibody specifically detects UBTF in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Recombinant UBTF antibody (pSer484) (ABIN7879748)

Target: UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This UBTF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 32U21

Product Details anti-UBTF Antibody

Binding Specificity: pSer484

Purpose: Phospho-UBF1 (Ser484) Antibody / Upstream-binding factor 1

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-UBF1 (S484) was used as the immunogen for the Phospho-UBF1 (Ser484) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-UBF1 (Ser484) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-UBF1 (Ser484) antibody at -20oC.

Target Details for UBTF

Target: UBTF (Upstream Binding Transcription Factor, RNA Polymerase I (UBTF))

Alternative Name: UBF1

Background: Phospho-UBF1 (Ser484) antibody recognizes upstream binding factor 1 phosphorylated at serine 484, a modification critical for ribosomal RNA transcription. UBF1 is a nucleolar transcription factor that binds rDNA promoters and recruits RNA polymerase I machinery to initiate rRNA synthesis. Phosphorylation at Ser484 is mediated by signaling kinases such as ERK and enhances UBF1 activity, thereby linking growth factor signaling to ribosome biogenesis.

Research employing Phospho-UBF1 (Ser484) antibody has highlighted its role in cell growth and proliferation. Enhanced UBF1 phosphorylation correlates with increased ribosome production in rapidly dividing cells, including cancer cells, where heightened protein synthesis supports tumor growth. Conversely, reduced phosphorylation is associated with decreased rRNA transcription during cellular stress or growth arrest, making Ser484 phosphorylation a sensitive marker of nucleolar activity.

Antibodies specific for phospho-UBF1 at Ser484 are useful in assays such as western blot, immunofluorescence, and chromatin immunoprecipitation. These reagents allow researchers to study how signaling pathways regulate ribosome synthesis and how nucleolar activity adapts to physiological conditions. Clone-based validation ensures that detection is specific to the phosphorylated form rather than total UBF1.

NSJ Bioreagents offers this Phospho-UBF1 (Ser484) antibody to aid in research on transcriptional regulation, signal transduction, and cancer biology. Alternate names include upstream binding factor 1 antibody, nucleolar transcription factor 1 antibody, and NOR-90 antibody.

UniProt: P17480