Recombinant PKC antibody (pSer729)

Catalog No. ABIN7879770

The Rabbit Monoclonal anti-PKC antibody is suitable to detect PKC in samples from Human and Rat. It has been validated for WB.

Quick Overview for Recombinant PKC antibody (pSer729) (ABIN7879770)

Target: PKC (Protein Kinase C (PKC))

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PKC antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 32P75

Product Details anti-PKC Antibody

Binding Specificity: pSer729

Purpose: Phospho-PKC (Ser729) Antibody / PRKCA/PRKCB/PRKCE

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Phospho-PKC (S729) was used as the immunogen for the Phospho-PKC (Ser729) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-PKC (Ser729) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-PKC (Ser729) antibody at -20oC.

Target Details for PKC

Target: PKC (Protein Kinase C (PKC))

Alternative Name: PKC

Background: Phospho-PKC (Ser729) antibody detects protein kinase C when phosphorylated at serine 729. Protein kinase C belongs to a family of serine threonine kinases encoded by multiple genes including PRKCA, PRKCB, and PRKCG. Phosphorylation at Ser729 occurs within the hydrophobic motif of PKC alpha and related isoforms, a modification essential for stability and full catalytic activity. This phosphorylation is typically mediated by kinases such as PDK1 and mTORC2, which regulate PKC maturation and downstream signaling capacity.

Phospho-PKC (Ser729) antibody is widely applied in cancer biology, signal transduction, and neuroscience. PKC enzymes regulate cell proliferation, differentiation, apoptosis, and cytoskeletal remodeling. Phosphorylation at Ser729 serves as a marker for mature active PKC isoforms and is required for proper subcellular localization and substrate phosphorylation. By detecting phospho Ser729, researchers can measure activation status of PKC pathways and how they change in disease states.

Western blot assays reveal distinct phospho PKC bands corresponding to activated isoforms. Immunohistochemistry maps Ser729 phosphorylation in tissues such as brain, heart, and tumors. Immunofluorescence highlights dynamic translocation of PKC from cytosol to plasma membrane or other compartments upon stimulation. These methods allow precise characterization of PKC activation at the molecular and cellular level.

PKC activity is critical for oncogenic signaling. Dysregulation of phosphorylation at Ser729 is associated with altered PKC stability and oncogenic transformation. Certain cancers show reduced PKC activity due to mutations preventing proper phosphorylation, while others display hyperactivation that promotes tumor growth. By applying Phospho-PKC (Ser729) antibody, scientists can study how phosphorylation events regulate tumor biology and therapeutic response.

PKC enzymes are also central to neuronal signaling and plasticity. Phosphorylation at Ser729 supports synaptic transmission, long term potentiation, and memory formation. Altered PKC activity has been implicated in neurodegenerative diseases such as Alzheimer disease and Parkinson disease. With phospho specific antibody tools, researchers can dissect how PKC phosphorylation influences brain function and pathology.

In cardiology, PKC phosphorylation regulates cardiac hypertrophy, contractility, and response to ischemia. The Ser729 site provides a biomarker for assessing PKC function in cardiac stress and adaptation. This makes Phospho-PKC (Ser729) antibody relevant not only for molecular signaling studies but also for translational research into heart disease.

Phospho-PKC (Ser729) antibody from NSJ Bioreagents provides strong specificity for detecting active phosphorylated PKC isoforms. Its reliable performance across multiple applications ensures accurate monitoring of kinase activity in health and disease.

UniProt: Q02156

Pathways: TCR Signaling, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Phototransduction