Recombinant Phospholipase C gamma 1 antibody (pTyr1253)

Catalog No. ABIN7879825

The Rabbit Monoclonal anti-Phospholipase C gamma 1 antibody (Clone 32P05) (ABIN7879825) specifically detects Phospholipase C gamma 1 in WB. The antibody is reactive with Human samples.

Quick Overview for Recombinant Phospholipase C gamma 1 antibody (pTyr1253) (ABIN7879825)

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Phospholipase C gamma 1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 32P05

Product Details anti-Phospholipase C gamma 1 Antibody

Binding Specificity: pTyr1253

Purpose: Phospho-Phospholipase C gamma 1 (Tyr1253) Antibody / PLCG1

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human phospho-Phospholipase C gamma 1/PLC-gamma-1 (Y1253) was used as the immunogen for the Phospho-Phospholipase C gamma 1 (Tyr1253) antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Phospho-Phospholipase C gamma 1 (Tyr1253) antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the Phospho-Phospholipase C gamma 1 (Tyr1253) antibody at -20oC.

Target Details for Phospholipase C gamma 1

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Alternative Name: Phospholipase C gamma 1

Background: Phospho-Phospholipase C gamma 1 (Tyr1253) antibody targets phospholipase C gamma 1 (PLCG1) when phosphorylated at tyrosine 1253. PLCG1 is an essential enzyme in intracellular signal transduction, catalyzing the hydrolysis of phosphatidylinositol 4,5-bisphosphate into diacylglycerol and inositol 1,4,5-trisphosphate. These second messengers trigger activation of protein kinase C and mobilization of intracellular calcium, respectively, initiating diverse downstream cellular responses.

Phosphorylation of PLCG1 at tyrosine 1253 is mediated by receptor tyrosine kinases and non-receptor tyrosine kinases. This phosphorylation event enhances catalytic activity and contributes to cell proliferation, differentiation, migration, and survival. Dysregulation of PLCG1 phosphorylation is implicated in oncogenesis, autoimmune disease, and vascular disorders.

Phospho-Phospholipase C gamma 1 (Tyr1253) antibody provides precise detection of the phosphorylated form, enabling researchers to evaluate signaling activation downstream of growth factor receptors such as EGFR, PDGFR, and VEGFR. It is commonly applied in studies involving cancer biology, immune receptor signaling, and angiogenesis.

This antibody is highly suitable for methods such as western blot, immunohistochemistry, immunofluorescence, and flow cytometry. By differentiating phosphorylated PLCG1 from total protein levels, it provides insight into dynamic cellular signaling events.

PLCG1 phosphorylation is especially important in cancer biology, where hyperactivation of growth factor signaling drives uncontrolled proliferation. Monitoring tyrosine 1253 phosphorylation provides mechanistic understanding of oncogenic transformation and therapeutic responses to kinase inhibitors. In immunology, PLCG1 phosphorylation regulates antigen receptor signaling, impacting T cell and B cell responses.

Phospho-Phospholipase C gamma 1 (Tyr1253) antibody from NSJ Bioreagents is a valuable reagent for researchers studying tyrosine kinase signaling, cell growth regulation, and immune function. Its phospho-specific recognition ensures accurate assessment of pathway activation in health and disease.

UniProt: P19174

Pathways: RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling