Recombinant Actin antibody

Catalog No. ABIN7879872

The Mouse Monoclonal anti-Actin antibody is suitable to detect Actin in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for Recombinant Actin antibody (ABIN7879872)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: RMSA-953

Product Details anti-Actin Antibody

Purpose: Muscle Actin Antibody (Skeletal/Cardiac/Smooth) (azide and preservative free)

Purification: Protein A/G affinity

Immunogen: Recombinant protein was used as the immunogen for the Muscle Actin antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Muscle Actin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Aliquot the Muscle Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: Muscle Actin

Background: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

UniProt: P62736

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development