Crossover junction endonuclease EME1 (EME1) antibody

Catalog No. ABIN7880514

The Mouse Monoclonal anti-Crossover junction endonuclease EME1 antibody is suitable to detect Crossover junction endonuclease EME1 in samples from Human. It has been validated for WB and IHC (p).

Quick Overview for Crossover junction endonuclease EME1 (EME1) antibody (ABIN7880514)

Target: Crossover junction endonuclease EME1 (EME1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MTA31 7H2-1

Product Details

Purpose: EME1 Antibody

Purification: Protein G affinity chromatography

Immunogen: His-tagged recombinant Eme1 of human origin was used as the immunogen for the EME1 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the EME1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the EME1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details

Target: Crossover junction endonuclease EME1 (EME1)

Alternative Name: EME1

Background: Essential meiotic endonuclease 1 (Eme1), a member of the Eme1/Mms4 family, associates with Mus81 to constitute a heterodimeric endonuclease that has been implicated in mitotic and meiotic recombination in eukaryotes. The Mus81-Eme1 complex cleaves branched DNA structures, especially those arising during stalled DNA replication such as replication forks and 3 DNA flaps. When purified from yeast, this complex cleaves synthetic Holliday junctions into linear duplex DNA. These findings provide compelling evidence that Mus81-Eme1 complexes are essential elements of the eukaryotic nuclear Holliday junction resolvase. Eme1 may also be required in mitosis for the processing of collapsed replication forks. Eme1 is typically localized to the nucleolus and is recruited to regions of DNA damage in S phase cells.

UniProt: Q96AY2