INSIG1 antibody

Catalog No. ABIN7881180

The Rabbit Polyclonal anti-INSIG1 antibody is suitable to detect INSIG1 in samples from Mouse and Rat. It has been validated for WB and FACS.

Quick Overview for INSIG1 antibody (ABIN7881180)

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INSIG1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-INSIG1 Antibody

Purpose: Insig1 Antibody / Insulin-induced gene 1

Sequence: MPRLHDHVWS YPSA

Purification: Affinity purified

Immunogen: Amino acids MPRLHDHVWSYPSA from the rat protein were used as the immunogen for the Insig1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Insig1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Insig1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for INSIG1

Target: INSIG1 (Insulin Induced Gene 1 (INSIG1))

Alternative Name: Insig1

Background: Insulin induced gene 1, also known as INSIG1, is a protein which in humans is encoded by the INSIG1 gene. This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants.

UniProt: Q08755

Pathways: ER-Nucleus Signaling