Recombinant IQGAP2 antibody

Catalog No. ABIN7881327

This Rabbit Monoclonal antibody specifically detects IQGAP2 in WB and IHC. It exhibits reactivity toward Human and Mouse.

Quick Overview for Recombinant IQGAP2 antibody (ABIN7881327)

Target: IQGAP2 (IQ Motif Containing GTPase Activating Protein 2 (IQGAP2))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This IQGAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 29I69

Product Details anti-IQGAP2 Antibody

Purpose: IQGAP2 Antibody / IQ motif containing GTPase activating protein 2

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human IQGAP2 was used as the immunogen for the IQGAP2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the IQGAP2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the IQGAP2 antibody at -20oC.

Target Details for IQGAP2

Target: IQGAP2 (IQ Motif Containing GTPase Activating Protein 2 (IQGAP2))

Alternative Name: IQGAP2

Background: IQGAP2 antibody detects IQ motif containing GTPase activating protein 2, encoded by the IQGAP2 gene. IQ motif containing GTPase activating protein 2 belongs to the IQGAP family of scaffolding proteins that interact with Rho family GTPases, actin filaments, and signaling molecules. It plays an important role in cytoskeletal organization, cell adhesion, and intracellular signaling. IQGAP2 antibody provides researchers with a means to study its contribution to cytoskeletal regulation, liver physiology, and cancer biology.

Unlike its homolog IQGAP1, which promotes oncogenic signaling, IQGAP2 functions primarily as a tumor suppressor. Research with IQGAP2 antibody has shown that loss of IQGAP2 expression is frequent in hepatocellular carcinoma and gastric cancer, where it contributes to uncontrolled proliferation and reduced cell adhesion. By interacting with Rho family GTPases, IQGAP2 regulates actin dynamics and stabilizes adherens junctions, helping maintain epithelial integrity. Loss of this regulation increases motility and invasion, linking IQGAP2 deficiency to metastatic progression.

IQGAP2 is also important in liver biology. Studies using IQGAP2 antibody have demonstrated that knockout mice develop spontaneous hepatocellular carcinoma, confirming its tumor suppressor role. In normal liver tissue, IQGAP2 interacts with beta catenin and other signaling molecules to regulate hepatocyte growth and polarity. Dysregulation of this protein contributes to abnormal signaling through pathways such as Wnt and PI3K AKT, reinforcing its significance in liver disease and cancer.

Beyond the liver, IQGAP2 contributes to immune regulation and cytoskeletal remodeling in other tissues. It has been implicated in platelet activation, immune synapse formation, and gastric mucosal stability. Research with IQGAP2 antibody continues to expand understanding of its roles in diverse systems. Importantly, reduced expression of IQGAP2 in tumors is associated with poor prognosis, making it a potential biomarker for patient stratification.

IQGAP2 antibody is applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects expression in liver and epithelial cell lines, while immunohistochemistry highlights its localization at cell junctions in tissues. Immunofluorescence reveals its colocalization with actin and signaling molecules, providing insights into cytoskeletal regulation. These methods ensure that IQGAP2 antibody is valuable for both fundamental and translational research.

By providing validated IQGAP2 antibody reagents, NSJ Bioreagents supports research into tumor suppression, cytoskeletal regulation, and liver cancer. Detection of IQ motif containing GTPase activating protein 2 helps researchers explore how scaffold proteins integrate signaling and structural networks in health and disease.

UniProt: Q13576