Recombinant IQGAP2 antibody

Catalog No. ABIN7881328

The Rabbit Monoclonal anti-IQGAP2 antibody is suitable to detect IQGAP2 in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant IQGAP2 antibody (ABIN7881328)

Target: IQGAP2 (IQ Motif Containing GTPase Activating Protein 2 (IQGAP2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This IQGAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 30I08

Product Details anti-IQGAP2 Antibody

Purpose: IQGAP2 Antibody / IQ motif containing GTPase activating protein 2

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human IQGAP2 was used as the immunogen for the IQGAP2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the IQGAP2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the IQGAP2 antibody at -20oC.

Target Details for IQGAP2

Target: IQGAP2 (IQ Motif Containing GTPase Activating Protein 2 (IQGAP2))

Alternative Name: IQGAP2

Background: IQGAP2 antibody detects IQ motif containing GTPase activating protein 2, encoded by the IQGAP2 gene. IQGAP2 belongs to a family of scaffolding proteins that integrate cytoskeletal dynamics, cell adhesion, and intracellular signaling. Unlike classical GTPase activating proteins, IQGAPs stabilize the active GTP bound form of small GTPases such as Rac1 and Cdc42. Through its IQ motifs, calponin homology domain, and RasGAP related domain, IQGAP2 coordinates actin reorganization, cell polarity, and signaling cascades.

IQGAP2 antibody is widely used in cancer biology, hepatology, and cytoskeletal research. IQGAP2 is highly expressed in liver, stomach, and testis. It regulates actin dynamics and adherens junctions by interacting with beta catenin, E cadherin, and F actin. Altered expression of IQGAP2 has been linked to hepatocellular carcinoma and gastric cancer, where loss of IQGAP2 promotes tumorigenesis. By detecting IQGAP2, researchers can study its tumor suppressor functions and its role in epithelial tissue integrity.

Western blot assays with IQGAP2 antibody detect protein bands in hepatic and epithelial tissues. Immunohistochemistry highlights expression in liver lobules and gastric mucosa, while immunofluorescence reveals cytoplasmic localization at cell junctions and actin filaments. ELISA enables quantification of IQGAP2 expression across experimental samples.

IQGAP2 participates in multiple signaling pathways, including Wnt, MAPK, and PI3K AKT. Its ability to bind both actin and signaling proteins positions it as a key scaffold at the plasma membrane. In cancer, IQGAP2 expression is frequently downregulated, contrasting with IQGAP1, which is often upregulated. This differential regulation underscores the unique tumor suppressive properties of IQGAP2. By applying IQGAP2 antibody, scientists can investigate how cytoskeletal regulation intersects with cancer biology and metabolic homeostasis.

IQGAP2 also plays roles in immune signaling and reproduction. In immune cells, it modulates cytoskeletal dynamics during migration and activation. In testis, IQGAP2 expression suggests involvement in spermatogenesis and germ cell polarity. These diverse roles make IQGAP2 antibody a valuable reagent for broad research areas beyond oncology.

IQGAP2 antibody from NSJ Bioreagents provides dependable specificity for studying cytoskeletal scaffolding and tumor suppression. Its strong performance across techniques ensures accurate detection of IQGAP2 in multiple research contexts.

UniProt: Q13576