HLA-B antibody

Catalog No. ABIN7881527

The Mouse Monoclonal anti-HLA-B antibody (Clone SPM420) (ABIN7881527) specifically detects HLA-B in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for HLA-B antibody (ABIN7881527)

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-B antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: SPM420

Product Details anti-HLA-B Antibody

Purpose: HLA-B Antibody (MHC Class I)

Purification: Protein A/G affinity

Immunogen: Normal human peripheral blood lymphocytes were used as the immunogen for the HLA-B antibody.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal dilution of the HLA-B antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the HLA-B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for HLA-B

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Alternative Name: HLA-B

Background: HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.

UniProt: P01889

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response