Nkx2-2 antibody

Catalog No. ABIN7881814

The Mouse Monoclonal anti-Nkx2-2 antibody is suitable to detect Nkx2-2 in samples from Human. It has been validated for IHC (p).

Quick Overview for Nkx2-2 antibody (ABIN7881814)

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nkx2-2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM564

Product Details anti-Nkx2-2 Antibody

Purpose: NKX2.2 Antibody

Purification: Protein G affinity chromatography

Immunogen: Human recombinant protein was used as the immunogen for this NKX2.2 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: The optimal dilution of the NKX2.2 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the NKX2.2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Nkx2-2

Target: Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

Alternative Name: NKX2.2

Background: Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors.

UniProt: O95096

Pathways: Dopaminergic Neurogenesis