Recombinant PSMC4 antibody

Catalog No. ABIN7882066

The Rabbit Monoclonal anti-PSMC4 antibody is suitable to detect PSMC4 in samples from Human, Mouse and Rat. It has been validated for WB, IF and ICC.

Quick Overview for Recombinant PSMC4 antibody (ABIN7882066)

Target: PSMC4 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 4 (PSMC4))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PSMC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 31P63

Product Details anti-PSMC4 Antibody

Purpose: PSMC4 Antibody / 26S proteasome regulatory subunit 6B / TBP-7

Purification: Affinity chromatography

Immunogen: A synthesized peptide derived from human Tbp7 was used as the immunogen for the PSMC4 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PSMC4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the PSMC4 antibody at -20oC.

Target Details for PSMC4

Target: PSMC4 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 4 (PSMC4))

Alternative Name: PSMC4

Background: PSMC4 antibody recognizes the 26S proteasome regulatory subunit 6B, also known as TBP-7, a protein encoded by the PSMC4 gene. This subunit is a member of the AAA ATPase family and forms part of the 19S regulatory particle of the 26S proteasome. The proteasome is the major protein degradation machinery in eukaryotic cells, responsible for removing damaged, misfolded, or short-lived regulatory proteins through ubiquitin-mediated proteolysis. PSMC4 antibody provides researchers with a key tool to study proteasomal structure and function, particularly in pathways linked to protein turnover, cell cycle regulation, and stress responses.

The 26S proteasome consists of a 20S catalytic core and a 19S regulatory complex. PSMC4 is one of six ATPase subunits within the base of the 19S regulator. These ATPases act as a molecular motor, unfolding ubiquitinated substrates and translocating them into the catalytic chamber for degradation. The role of PSMC4 is critical for energy-dependent substrate processing. Detection with PSMC4 antibody supports studies on proteasome assembly, activity, and substrate specificity.

Dysfunction of proteasome regulation has been linked to neurodegenerative diseases, cancer, and autoimmune disorders. Mutations or altered expression of proteasome subunits, including PSMC4, can disrupt proteostasis and lead to the accumulation of toxic protein aggregates. PSMC4 antibody has therefore been used to examine proteasome activity in conditions such as Parkinson disease, Huntington disease, and multiple myeloma. In cancer biology, changes in proteasome function influence cell proliferation and apoptosis, making it a therapeutic target.

PSMC4 antibody is suitable for use in western blotting, immunohistochemistry, and immunoprecipitation. Western blotting reveals subunit expression within the proteasome complex, while immunohistochemistry highlights localization in tissues with high proteolytic activity, such as liver and brain. Immunoprecipitation using PSMC4 antibody aids in proteasome purification and analysis of protein-protein interactions.

Researchers studying pathways of ubiquitin-mediated degradation, antigen presentation, and stress responses rely on antibodies such as PSMC4 antibody to track proteasome composition and function. NSJ Bioreagents offers validated reagents for investigating this essential protein degradation system across a wide range of experimental applications.

UniProt: P43686

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway