DBH antibody

Catalog No. ABIN7884240

This Mouse Monoclonal antibody specifically detects DBH in WB, IHC, ELISA and ICC. It exhibits reactivity toward Rat.

Quick Overview for DBH antibody (ABIN7884240)

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This DBH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Clone: DBH41

Product Details anti-DBH Antibody

Purpose: Dopamine Beta Hydroxylase (DBH) Mouse Monoclonal Antibody (DBH41)

Specificity: This antibody recognizes This antibody specifically recognizes two bands of molecular weight 70 and 75 kDa, corresponding to the soluble and membrane bound forms of the enzyme, respectively. Due to the high sensitivity of the DBH antibody, the presence of the enzyme can be immunologically detected even in areas where only scattered DBH-containing fibers are present. DBH antibody specifically stains DBH-containing neurons and fibers in the rat central nervous system (CNS). It exhibits no cross-reactivity with either mouse, human, rabbit, guinea pig, cat or bovine DBH

Immunogen: Rat DBH

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 2 ml: IHC and ICC: 1:30 - 1:50. Optimal dilution has to be determined by the user.

Buffer: Lyophilized tissue culture supernatant

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for DBH

Target: DBH (Dopamine beta-Hydroxylase (Dopamine beta-Monooxygenase) (DBH))

Alternative Name: Dopamine Beta Hydroxylase

Background:

Dopamine beta-monooxygenase Dopamine Beta Hydroxylase, Dopamine-hydroxylase, DBH, oxygenase, anti-Dopamine Beta Hydroxylase, anti-Dopamine-hydroxylase, anti-DBH, oxygenase

Studying the monoaminergic system has been of great interest and importance since dopamine, noradrenaline and adrenaline are the neurotransmitters involved in multiple physiological and pathophysiological conditions. DBH is the enzyme responsible for the biosynthetic hydroxylation of dopamine to noradrenaline.

Pathways: Carbohydrate Homeostasis