GHRH antibody (AA 1-29)

Catalog No. ABIN7884242

The Sheep Polyclonal anti-GHRH antibody (ABIN7884242) specifically detects GHRH in IHC, RIA and Imm. The antibody is reactive with Rat samples.

Quick Overview for GHRH antibody (AA 1-29) (ABIN7884242)

Target: GHRH (Growth Hormone Releasing Hormone (GHRH))

Reactivity: Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This GHRH antibody is un-conjugated

Application: Immunohistochemistry (IHC), Radioimmunoassay (RIA), Immunogen (Imm)

Product Details anti-GHRH Antibody

Binding Specificity: AA 1-29

Purpose: Growth Hormone-Releasing Hormone Sheep Polyclonal Antibody

Specificity: The antibody recognizes the rat GHRH and it shows no cross-reactivity with SRIF-14, SRIF-28 rGH, LH, FSH, PRL calcitonin, insulin, glucagon ovine CRF, secretin, vasoactive intestinal peptide, and cholecystokinin-8

Immunogen: synthetic rat GHRH (1-29)NH2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 50 μL: IHC: 1:15000, In vivo Passive Immunization: neat serum, RIA: In a RIA for rGRF-(1043), at a final dilution of 1: 785,000, this antiserum binds 35 % of [125I]-rGRF and can detect 7pg/assay tube. Optimal dilution has to be determined by the user.

Buffer: Lyophilized serum

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for GHRH

Target: GHRH (Growth Hormone Releasing Hormone (GHRH))

Alternative Name: Growth Hormone-Releasing Hormone

Background:

GHRH, Somatocrinin, Somatoliberin, GH-RH, Growth hormone-releasing factor (GHRF or GRF), Somatotropin-releasing hormone (SRH), Somatotropin-releasing factor (SRF), Somatorelin

GHRH belongs to the glucagon family produced in the hypothalamus. It is essential for normal linear growth and has important metabolic effects throughout life. GHRH stimulates GH production and release by binding to the GHRH Receptor (GHRHR) on cells in the anterior pituitary. GHRH is released from neurosecretory nerve terminals of arcuate neurons, and is carried by the hypothalamo-hypophyseal portal system to the anterior pituitary gland, where it stimulates growth hormone(GH) secretion by stimulating the growth hormone-releasing hormone receptor.

Pathways: Hormone Transport, Hormone Activity, cAMP Metabolic Process