Adenosine A3 Receptor antibody (3rd Intracellular Loop)

Catalog No. ABIN7884726

This Rabbit Polyclonal antibody specifically detects Adenosine A3 Receptor in WB, IF, IHC and IC. It exhibits reactivity toward Human and Mouse.

Quick Overview for Adenosine A3 Receptor antibody (3rd Intracellular Loop) (ABIN7884726)

Target: Adenosine A3 Receptor (ADORA3)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adenosine A3 Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunochromatography (IC)

Grade: Carrier-free

Product Details anti-Adenosine A3 Receptor Antibody

Binding Specificity: 3rd Intracellular Loop, AA 216-230

Purpose: A Rabbit Polyclonal Antibody to Adenosine A3 Receptor

Predicted Reactivity: Rat,mouse - 14,16 amino acid residues identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KETGAFYGREFKTAK, corresponding to amino acid residues 216-230 of human A3AR

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:100

IHC: 1:50

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234639)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Adenosine A3 Receptor

Target: Adenosine A3 Receptor (ADORA3)

Alternative Name: ADORA3

Background:

Synonyms: ADORA3, A3AR, Adenosine A3R

Description: Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its action via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). The various adenosine receptors can be distinguished on the basis of their distinct molecular structures, distinct tissue distributions, and differential selectivity for adenosine analogs.1-4 All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important in binding G proteins.1-3Expression of A3AR has been reported in the brain, kidney, liver, and heart. It plays a role in modulation of cerebral ischemia, asthma, and cell growth. In addition, recent studies have established a cardioprotective role for A3AR.5 Expression of A3AR was reported to be elevated in cancerous tissues as well as in auto-immune inflammatory diseases.6,7 A patent has been filed for the use of A3AR and A3AR agonist as a diagnostic marker for therapeutic treatments of cancer and other diseases.

Gene ID: 140

UniProt: P0DMS8

Pathways: Hormone Transport, cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process