ADCY3 antibody (3rd Extracellular Loop)

Catalog No. ABIN7884730

The Rabbit Polyclonal anti-ADCY3 antibody is suitable to detect ADCY3 in samples from Human, Rat and Mouse. It has been validated for WB, IHC, IF, FACS, IC and LCI.

Quick Overview for ADCY3 antibody (3rd Extracellular Loop) (ABIN7884730)

Target: ADCY3 (Adenylate Cyclase 3 (ADCY3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADCY3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunochromatography (IC), Live Cell Imaging (LCI)

Grade: Carrier-free

Product Details anti-ADCY3 Antibody

Binding Specificity: 3rd Extracellular Loop, AA 285-299

Purpose: A Rabbit Polyclonal Antibody to Adenylate Cyclase Type III

Predicted Reactivity: Mouse,human - identical

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KHVADEMLKDMKKDE, corresponding to amino acid residues 285-299 of rat ADCY3

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: 1:50

IHC: 1:400

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234646)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW)

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Preservative: Without preservative

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for ADCY3

Target: ADCY3 (Adenylate Cyclase 3 (ADCY3))

Alternative Name: Adenylate cyclase type 3

Background:

Synonyms: Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3

Description: Adenylyl cyclases (ACs) are a family of enzymes that synthesize cAMP, upon stimulation. cAMP is an important second messenger, which regulates carbohydrate, lipid, protein, and nucleic acid metabolism as well as synaptic transmission, ion channel function, and transcription in neurons1. There are nine closely related isoforms of ACs (AC1-9) in mammals. All AC isoforms share a primary structure consisting of two hydrophobic regions which comprise six transmembrane helices and three large cytoplasmic domains. The basic catalytic unit of a membrane-bound AC molecule consists of the C1a and C2 domains. The N-terminus domains of ACs are highly variable, and might play a regulatory role2.Adenylyl cyclase type 3 (AC3) is widely expressed in the olfactory tissue, human cell line HEK-293, bovine brain, spinal cord, adrenal medulla, adrenal cortex, heart atrium, aorta, lung and retina3.Several studies have suggested that AC3 may play an important role in the regulation of glucose metabolism and body weight. It was found that overexpression of AC3 mRNA is involved in pathophysiological metabolic processes, such as type 2 diabetes4. In addition, a recent study demonstrated the association of AC3 with gastric cancer as well as its tumorigenic potentials5.Furthermore, AC3-knockout mice showed peripheral and behavioral anosmia6.

Gene ID: 64508

UniProt: P21932

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma